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KMID : 0351219930250020151
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Korean Journal of Infectious Diseases
1993 Volume.25 No. 2 p.151 ~ p.157
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Detection of cytomegalovirus DNA by Polymerase Chain Reaction in Renal Tissues from Various Glomerulonephritis
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Abstract
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Cytomegalovrus (CMV) has been suspected to be involved in the pathogenesis of IgA nephropathy (IgA N). However, the presence of CMV in renal tissue of IgA N remains to be the subject of controversy. To elucidate the presence of CMV in IgA N and
other
glomerulonephritis (GN), polymerase chain reaction (PCR) which specifically amplified a 159 bp fragment of immediate early gene of CMV and indirect immunofluorescence (IF) with monoclonal antibody to early and late gene of CMV were performed in
paraffin-embedded tissues from 10 IgA N and 14 non-IgA GNs. CMV DNA was detected in 6 of 10 IgA N tissues and 10 of 14 other GNs by PCR-Southern blot hybridization, whereas IF failed to show the presence of CMV antigen in all renal tissues. the
nonspecific presence of CMV DNA in various kinds of GNs including IgA N would suggest that CMV is not specifically associated with the pathogenesis of IgA N in endemic areas of CMV infection.
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KEYWORD
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